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Mineral Processing Equipment : Ball mill mineral processing assays for superoxide anion - A type of mining equipment that can trigger the development and change of the beneficiation technology industry. The main core machines are ball mills, rod mills, flotation machines, magnetic separators, etc.
1 The dismutation of superoxide anion to generate hydrogen peroxide. 2 The Haberâ€Weiss reaction responsible for generating the pernicious hydroxyl radical OH from hydrogen peroxide and superoxide in the presence of transition metal Fe 2. 3 The hydroxyl radical then participates in a hydrogen abstraction reaction with a ...
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2.6.3. Assay for Superoxide Anion Free Radical Scavenging. Superoxide anion radical scavenging activity was determined by the 24-iodiphenyl-34-nitrophenyl-5-phenyltetrazolium chloride NBT method. An antioxidant kit was used as previously reported . The superoxide anion free radicals generated by the xanthine-xanthine oxidase system reacted ...
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2.7. Measurement of Superoxide Anion Radical Reduction Assay from HL-60 Cell Line. Human promyelocytic leukemia cell line HL-60 purchased from the American Type Culture Collection ATCC USA was cultured in RPMI 1640 with 10 fetal bovine serum and 1 penicillin-streptomycin at 37 C in humidified atmosphere containing 5 CO 2.
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3.2. Determination of Total Phenolic Content. Figure 1 shows the total phenolic content in the samples of different leafy vegetable and fruit crops grown in Tower Garden aeroponic systems and in the soil. Among the leafy vegetables the highest phenolic content was found in chard 57.73 mg GAEg dry wt. in FG and 53.45 GAEg dry weight in AG followed by basil red kale and parsley.
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3.3.4. Superoxide anion-scavenging activity . The results of superoxide radical scavenging assay are described in Figure 1. All the samples were found to be very good O 2 - radical scavengers with the range of 66-90 and the scavenging ability was increased with the sample after processing.
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5.4 Results of the insulin reduction assay used to determine the biological activity of recombinant Fasciola hepatica thioredoxin..... 91 5.5 Effects of phorbol 12-myristate 13-acetate PMA and recombinant Fasciola hepatica thioredoxin TRX on the production of superoxide anion O2- on activated mouse peritoneal macrophages
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6. Resuspend the cells in 100 l of Superoxide Anion assay medium. Preparing the Samples for the Superoxide Anion Assay Prepare the supernatant of the cells suspected to contain SOD using to an appropriate protocol. The total protein content of the cell supernatant should be 0.8-40.0 g in a final volume of 5-10 l.
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All the assays were carried out in triplicate. Determination of superoxide anion scavenging activity: The superoxide anion scavenging activity was assayed by measuring the inhibition of the autoxidation of pyrogallol using the method of Marklund and Marchland 1974 with a slightly modification. One mL of the sample solution 2 mgmL and 4.5 ...
Read MoreAntioxidant Assays and Techniques-Lecture Series. Hydroxyl Radical Scavenging Assay Antioxidant Assay and Techniques Lecture 1 FREE 00:03:00; Nitric Oxide Scavenging Assay Antioxidant Assay and Techniques Lecture 2 FREE 00:04:00; Superoxide Anion Radical Scavenging Assay Antioxidant Assay and Techniques Lecture 3 FREE 00:03:00; DPPH Assay Antioxidant Assay and Techniques ...
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Assay of antioxidant enzymes was performed by homogenizing leaf tissues at 15000 g for 20 min at 4ËšC with three volumes wv of an ice-cold extraction buffer 50 mM Tris-HCl pH 7.8 1 mM EDTA 1 mM MgCl2 and 1.5 ww polyvinyl pyrrolidone. The crude extract from the supernatant was used for the assay of enzyme activities.
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Aug 01 1978 Deactivation of superoxide anions may prevent their reaction with unsaturated fipids 12 30 although this reaction appears to be unfavorable 19 compared to the Fenton-like reaction 9 between superoxide anion and hydrogen peroxide. Superoxide anion reacts slowly with hydrogen peroxide to produce hy- droxyl radicals 11 20.
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Aug 19 2012 Zhang L Huang D Kondo M Fan E Ji H Kou Y Ou B 2009 Novel high-throughput assay for antioxidant capacity against superoxide anion. J Agric Food Chem 577:2661â€2667 CrossRef Google Scholar Zhou K Su L Yu L 2004 Phytochemical and antioxidant properties in …
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Background. The free radicals FR and reactive oxygen species ROS are produced through frequent physiological and biochemical processes in the human body as byproduct 12.ROS includes a number of chemically reactive molecules derived from oxygen such as hydrogen peroxide H 2 O 2 superoxide O 2- and hydroxyl radical OH- etc.Over production of such free radicals might leads to ...
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Both 2APT and 2APT-D6 inhibited collagen-dependent platelet aggregation adhesion thrombus formation superoxide anion generation and surface activation marker expression while responses to thrombin or adhesion to fibrinogen were not affected.
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Boubekri c.; koraichi b. solvent extraction effects on phytochemical profiles antioxidant activities and superoxide anion radical interactions of whole fruit pulp and skin of cucurbita pepo l. journal of fundamental and applied sciences v. 12 n. 3 p. 1204-1222 30 ago. 2020.
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Chalcopyrite or pyrite mineral of 100 g was dry-ground in a laboratory ball mill and the ... with pyrite can form superoxide anion O 2 ... in Mineral and Metals Processing vol. VI The ...
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Chalcopyrite or pyrite mineral of 100 g was dry-ground in a laboratory ball mill and the formation of hydrogen peroxide was studied when the dry-ground solids are mixed in water. The effect of water pH in which the solids are mixed on the formation of hydrogen …
Read MoreCiteSeerX - Document Details Isaac Councill Lee Giles Pradeep Teregowda: Various studies have been focussed on the protective and preventive effect of antioxidant activity on certain degenerative illnesses such as cardiovascular cancer neurological diseases cataracts and oxidative stress dysfunction. The present work was conducted to evaluate the antioxidant activity of processed dry ...
Read MoreDetailed protocols for simultaneous measurements of mitochondrial superoxide generation and apoptotic markers APC annexin V A35110 Assays for Apoptosisâ€Section 15.5 and SYTOX Green S7020 Nucleic Acid Stainsâ€Section 8.1 in human coronary artery endothelial cells by flow cytometry have been published by Mukhopadhyay and co-workers.
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During leukocytospermia the superoxide anion produced by leukocytes is an oxygen radical that along with high levels of inflammatory chemokines may be important for bacterial killing Rajasekaran et al 1995. Because vitamin E is an antioxidant the bacterialâ€killing mechanism of leukocytes may well be inhibited by large doses of vitamin E ...
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Feb 01 1988 Superoxide generated by pyrogallol reduces highly water-soluble tetrazolium salt to produce a soluble formazan: a simple assay for measuring superoxide anion radical scavenging activities of biological and abiological samples. Xu C Liu S …
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Feb 01 2007 The charged superoxide anion is a relatively short-lived radical which has limited membrane permeability. It is rapidly converted to H 2 O 2 by SOD and therefore assays for detection of O 2 − must compete for O 2 − as a substrate. Multiple assays and probes have been developed for low-level superoxide detection in nonphagocytic cells such ...
Read MoreHydroxyl and superoxide radicals have been implicated in a number of pathological conditions including ischemia reperfusion and aging. The superoxide anion Reactive oxygen speciesâ€Table 18.1 may also play a role in regulating normal vascular function.
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INTRODUCTION. Second only to the mitochondrial electron transport chain the most notable enzyme involved in reactive oxygen species ROS production is the NADPH oxidase of phagocytic leukocytes .It belongs to a family of enzymes which include NOX1 through to NOX5 that uses NADPH as an electron acceptor and catalyzes the one electron reduction of molecular oxygen to produce superoxide anion ...
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Jul 09 2018 The superoxide anion scavenging effects of YL and FY were investigated. Dose-dependent effects were observed containing the range of peptide concentrations from 0 to 1.0 mgml . YL and FY showed similar superoxide anion radical-scavenging activity 42.63 0.47 and 40.36 1.28 respectively at the concentration of 1.0 mgmL p 0
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Measurement of Superoxide Anion Scavenging Activity. Super-oxide radical formation was monitored by the nonenzymatic reduction of nitroblue tetrazolium NBT. Assays were performed as described by Robak and Gryglewsi 15 with minor modifications. NBT 0.50 mL of 156 M in 0.1 M potassium phosphate pH 6.8 was combined
Read MoreMisra HP Fridovich I 1972 The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. J. Bio Chem 247:3170-3175
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Nov 27 2020 Superoxide Dismutase is an oxidoreductase that catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. The enzyme protects the cell against ...
Read MoreOct 03 2020 Superoxide anion radical scavenging activity assay Mix 1.6 mL of sample with an equal volume of 50 mM Trisâ€HCl buffer pH 8.3. The mixture was incubated in a bath for 20 min then 0.8 mL of 1.5 mM pyrogallic acid was added.
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Rapid assay for testing superoxide anion radical scavenging activities to natural pigments by ultra-high performance liquid chromatography-diode-array detection method. Analytical Methods 2015 7 …
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